Metabolomic profiling directly from intact subcutaneous and visceral fat samples obtained from patients with obesity

This study investigates metabolic differences and similarities between subcutaneous and visceral adipose tissue in individuals with obesity using minimally invasive, intact-tissue metabolomics. Paired adipose tissue samples were collected during bariatric surgery and analyzed using solid-phase microextraction (SPME) coupled with complementary liquid chromatography–mass spectrometry platforms. Both targeted LC-MS/MS (Shimadzu triple quadrupole) and high-resolution LC-HRMS analyses were employed to characterize metabolite profiles related to energy metabolism, amino acid metabolism, redox balance, and inflammation. The dataset provides curated metabolomics data from human adipose tissue, including biological samples, pooled quality controls, and extraction blanks, enabling assessment of analytical reproducibility and background signals. By avoiding tissue homogenization and solvent-intensive extraction, this study offers a complementary perspective on bioavailable metabolites within native adipose tissue architecture. The data are intended to support reuse in comparative metabolomics, methodological evaluation of SPME-based sampling, and future meta-analyses of adipose tissue metabolism in obesity.