基于便携式荧光试纸条的水体抗生素比率荧光检测方法研究

This study aims to develop a novel, highly sensitive and selective method to address the ecological and human health risks posed by antibiotic pollution in aquatic environments.Zeolitic imidazolate framework-8(ZIF-8) was synthesized via a one-step method and systematically characterized using FT-IR,XRD,SEM,TEM,and XPS.A ratiometric fluorescence sensor was then constructed for the detection of levofloxacin(LFX).Under optimal conditions(ZIF-8 concentration: 25 μg/mL,pH 7.0,reaction time: 5 min),the sensor exhibited a linear detection range of 1～80 μmol/L for LFX,with a detection limit of 0.29 μmol/L.The recovery rate in real water samples was from 98.27% to 101.9%,with RSD≤2.99%,showing good agreement with HPLC results.Additionally, a smartphone-based portable test strip sensor was developed, enabling real-time detection in the range of 10～100 μmol/L.This study provides a new approach for designing ZIF-8-based fluorescence sensors and establishes a rapid detection method for trace antibiotic pollutants in environmental water.