Fetus-derived IGF2 matches placental development to fetal demand II [RNA-seq]

We used transcriptome analysis to identify genes transcriptionally disregulated in feto-placental endothelial cells from mutants with conditional deletion of Igf2 gene driven by the Tek-cre. RNA-seq in FACS-sorted feto-placental endothelial cells isolated from E16 Igf2ECKO mutants (n=4) and littermate controls (n=4)本研究采用转录组分析技术，鉴定由Tek-cre驱动的Igf2基因条件性敲除突变体的胎儿-胎盘内皮细胞中发生转录失调的基因。随后，对从E16（胚胎第16天）Igf2ECKO突变体（n=4）及其同窝对照（n=4）中分离得到的、经荧光激活细胞分选（FACS）纯化的胎儿-胎盘内皮细胞开展RNA测序（RNA-seq）。