ATOH8 and SMAD3 form a transcriptional complex to induce an anti-cell cycle signature and prevent cell cycle progression [ChIP-seq]

To identify ATOH8 and SMAD3 form a novel transcriptional complex, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq). Analyses of their genome occupancy profiling reveals that their genome-wide binding patterns are highly similar and they co-occupy a number of same genes. CCNE2 and CDK1 are their common downstream target genes. Overall design: ChIP-seq was generated to identify ATOH8 and SMAD3 transcriptional program in ATOH8 overexpressing A549.