The effect of buffer on median and maximum lifespan of H. polymorpha.
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*Cells were grown in the presence of ammonium sulphate as nitrogen source**Upon reaching the stationary phase the cells were kept in the same medium or collected by centrifugation and resuspended in buffer.The median lifespan is defined as time point when 50% of the cells survive, the maximum lifespan when 10% of the cells survive (Fig. 1A).
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2015-12-02



