RNA-seq study of SRSF2 over-expressing lung cancer cell lines

Immune checkpoints blockade therapy may have clinical symptoms of hyper-progressiveness (HP). The molecular mechanism of HP is still to be revealed. This study inspected transcriptome difference between clinical hyper-progressive samples to immunotherapy-response samples based public datasets. Spliceosome factors SRSF2 were found most enriched in neoantigen-related splicing events. Similar splicing pattern and increased cell invasion were found in two lung cancer cell lines after SRSF2 over-expressed.This study shows that dysregulation of SRSF2 -related splicing might be an important feature of immune hyper-progress occurrence. Overall design: SRSF2 over-expressing lung cancer cell lines compared to normal cell lines.Total RNA transcriptome from these samples was profiled by RNA-seq.