Investigation the number and structure of chromosomes in men from infertility couples: a massive prospective study

This retrospective study enrolled men from infertile couples at the Military Institute of Clinical Embryology and Andrology and Fertility Hospital of Hanoi between January 2020 and December 2023. A total of 18,584 male subjects were included. Karyotyping was performed to identify numerical and structural chromosomal abnormalities. Variations in heterochromatin length were also reported. In cases where karyotyping indicated a structural deletion on the Y chromosome, multiplex polymerase chain reaction (PCR) was used to detect Y chromosome microdeletions. Data were analyzed using STATA 16.0 software.