Effect of HIF-1alpha and HIF-2alpha on hypoxic response in Panc1 cells

The hypoxic response is influence by both the tissue of origin and local environmental influences, and mediated in large part by the hypoxia-inducible transcription factors HIF-1 and HIF-2. We looked at Panc1 cells, a pancreatic adenocarcinoma cell line, and compared the response to hypoxia in both regular tissue culture media and in pancreatic TIFM (tumor interstitial fluid media), and assessed the degree to which HIF-1 and HIF-2 knockout mediates changes in the hypoxia response. Overall design: RNA-sequencing of Panc1 control cells, HIF-1a knockout cells, and two independently derived clones of HIF-2a knockout cells, generated by CRISPR-Cas 9 technology. Samples are exposed to normoxia (20% O2)/regular media (DMEM + 10% FBS + 1% P/S), hypoxia (1% O2)/regular media, or hypoxia/pancreatic TIFM (described by Muir A et al., eLife 2023), for 48 hours.