Cololabis saira Genome sequencing and assembly. Cololabis saira

This project aims to provide an analytical basis for biology of the Pacific saury (Cololabis saira) by means of large-scale DNA data acquisition. Pacific saury is a commercially important small pelagic fish species in Asia. In this study, we conducted the first-ever whole genome sequencing of this species, with single molecule, real-time (SMRT) sequencing technology. The obtained high-fidelity (HiFi) long-read sequence data, which amount to ~30-folds of its haploid genome size that was measured with quantitative PCR (1.17 Gb), were assembled into contigs. Scaffolding with Hi-C reads yielded a whole genome assembly containing 24 chromosome-scale sequences, with a scaffold N50 length of 47.7 Mb. Protein-coding regions were predicted using transcriptome data, which resulted in 22,274 components. These resources are available at https://treethinkers.nig.ac.jp/saira/ and will assist various molecular-level studies in fishery science and comparative biology.