Additional file 4 of Horizontal transfer and proliferation of Tsu4 in Saccharomyces paradoxus

Multiple sequence alignment of Ty4 and Tsu4 elements in Saccharomyces sensu stricto species. Multiple sequence alignment of all full-length Ty4/Tsu4 elements from 12 strains of S. cerevisiae and S. paradoxus with PacBio assemblies from Yue et al. [13] plus all complete or nearly-complete Tsu4 elements identified in Saccharomyces WGS assemblies at NCBI (> 80% coverage and > 80% identity relative to the Tsu4 query sequence from S. uvarum). Fasta files of Ty4/Tsu4 sequences from all strains plus the Ty4 and Tsu4 query sequences were concatenated together and aligned using MAFFT (version 7.273-e; options: –thread 28) [52]. (TXT 335 kb)