Abundant polyadenylation of transcripts and precursor tRNAs in Mycobacterium tuberculosis upon depletion of Rv3907c, the mycobacterial CCA-adding enzyme

RNA-Seq results accompanying submission of a manuscript: "Depletion of CCA-adding enzyme in Mycobacterium tuberculosis leads to polyadenylation of transcripts and precursor tRNAs" describing the function of the Rv3907c gene product as a CCA-adding enzyme in Mycobacterium tuberculosis. Overall design: Next generation sequencing results are provided in three independent biological replicates for each strain growing in standard 7H9/10% OADC medium. The bacteria were grown to mid-logarythmic phase of growth, when they were collected and total RNA was isolated. The resulting RNA was subjected to ribodepletion with riboPools Pan-Bacteria (siTools Biotech GmbH,Planegg, Germany) and used for preparation of sequencing libraries,generated with a KAPA-stranded RNA Seq kit according to the manufacturer's protocol (Roche Diagnostics, Rotkreuz, Switzerland) or TruSeq small RNA library preparation kit (Illumina, San Diego, CA, USA). The resulting adapter-ligated, PCR-amplified cDNA libraries were subjected to sequencing on a NextSeq500 sequencer using the NextSeq500/550 v.2 sequencing kit (Illumina, San Diego, CA, USA), and approximately 5 to 10 million paired-end reads were sequenced for each sample for total RNA-Seq and 0.5-2.5mln were sequenced for small RNA libraries. VAHTS mRNA-seq V3 Library Prep Kit for Illumina® (Vazyme, Nanjing, China) with detailed manufacturer's protocol was applied to generate final sequencing libraries for the polyadenylation enriched RNA samples obtained from Mycolicibacterium smegmatis strains.