Profiles of expression HL-1 cardiomyocites cells stimulates with Insulin or aggregated LDL. Mus musculus

The murine HL-1 cardiomyocyte-derived cell line was generated by Dr. W.C. Claycomb (New Orleans, Louisiana, USA). Cells were maintained in Claycomb medium (Sigma-Aldrich) in plastic dishes coated with 12.5 g/ml fibronectin (Sigma-Aldrich) and 0.02 % gelatin, in a 5% CO2 atmosphere at 37 C (2). HL-1 cardiomyocytes were fasted with 0 % FBS for 12 h and then incubated with Insulin (100 nM, 2 h) (Sigma-Aldrich) or aggLDL (100 ug/mL, 8 h).