Compositions and methods for producing plants resistant to glyphosate herbicide
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family， e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system， wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically， compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells， plants explants， seeds and grain.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
