Proximity labeling in Arabidopsis seedlings expressing R-degron-Turbo vs. M-degron-Turbo vs. L-degron-Turbo untreated (DMSO control), treated with 10µM Bortezomib and treated with 1µM Concanamycin A.

Goal was to identify N-recognins for Arginine and Leucine by comparison of biotinylated proteins from 1 week old whole Arabidopsis seedlings constitutively expressing the bait constructs R-degron-Turbo or L-degron-Turbo. M-degron-Turbo served as a negative control. The plants were treated with 50µM biotin and either the proteasome inhibitor Bortezomib (10µM) or the inhibitor of vacuolar acidification Concanamycin A (1µM) or DMSO one hour before harvest.