Transcriptome of embryonic hearts with cardiomyocyte-specific deficiency of Pcbp1

We generated cardiomyocyte-specific Pcbp1 knockout mice, and observed ventricular non-compaction defects and abnormal cardiac morphogenesis at E12.5 and E16.5. Majority of homozygous mutant mice died shortly after birth. We sought to identify the transcriptome changes at E16.5 by Pcbp1-deficiency in cardiomyocytes. Overall design: We cross Pcbp1-Flox mice with Ctnt-Cre to generate cardiomyocyte-specific deletion of Pcbp1. On embryonic day (E) 16.5, cardiac ventricles from mutant mice and littermate controls were dissected, snap frozen and processed for RNA isolation. Genotype of individual embryos were confirmed by PCR from DNA isolated from yolk-sacs or tail tips. E16.5 heart RNA were processed for rRNA-depleted library construction and RNA-sequencing.