Extended-Spectrum Beta-Lactamase(ESBL)-producing E.coli from raw meats in Singapore markets

A total of 634 chilled and frozen meat samples (chicken n=213, pork n=216, beef n=205) were purchased from wet markets and five main chain supermarkets in Singapore during June 2017-October 2018. 97 supermarkets and 65 wet markets all over the island were involved in this project. Presumptive ESBL-producing E.coli isolates were selected on Brilliance TM CRE Agar (Thermo Scientific) and further confirmed by Double Disc Synergy Test (DDST). All confirmed ESBL-producing E.coli were sent for whole-genome sequencing (WGS) using illumina Hiseq2500 sequencer.