High Mobility Group protein HMGB2 is a Critical Regulator of Plasmodium Oocyst Development. Plasmodium yoelii

Two mice per group were infected with one million purified schizonts from the WT and two clones (B3 and D1) of the pyhmgb2 KO lines. Parasitemia reached 10 to 20 % and equivalent gametocytemia total RNA was extracted from the WT and the B3 and D1 clones of the KO parasite lines using the Trizol method on saponin lysed of parasites. Keywords: gene expression Overall design: Four samples analysed including dye-swap