Raw data for Fig 7

Contents:
These data relate to Fig.7 of Sampattavanich et al., Cell Systems (2018). Refer to accompanying Git repository for scripts used for Fig.7.
To use this data with our Git Repository:Placed these in the /rawdata/fixedcell folder.
Each cell line has three .MAT files for three proteins: FoxO3, ERK and AKT. Each single_Protein variable has the dimension of TimePoint x Row x Column.
Raw Index indicates growth factor types, each at 100 ng/mL: (1)EGF (2)IGF1 (3)FGF1 (4)HRG (5)HGF (6)EPR (7)BTC (8)Non-stimulated control
Column Index indicates drug pre-treatment condition: (1)DMSO control (2)AKTi(10uM) (3)MEKi(10uM) (4)AKTi(10uM)+MEKi(10uM)

Timepoint Index varies between cell lines184A1: 08-19-2013 (result 08-22-2013) including 13-timepoints: 0, 5, 10, 15, 20, 30, 45, 60, 90, 120, 180, 300, 480 minutes
MCF10A: 11-26-2013 (result 11-29-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
SKBR3: 10-22-2013 (result 10-25-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
BT-20: 10-22-2013 (result 10-25-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
MCF7: 11-05-2013 (result 11-09-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
T47D: 11-26-2013 (result 11-29-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
Hs578T: 11-05-2013 (result 11-08-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
MDA231: 11-26-2013 (result 11-29-2013) including 8 time points:0,0:15,0:30,1,1:30,2,3,4HRs.
HCC1806: 12-17-2013 (result 12-20-2013) including 8 time points: 0,0:15,0:30,1,1:30,2,3,4HRs.
Experimental Setup:

Cells were serum starved for 6 hours, and were then pre-treated with different inhibitor conditions for 1 hour. Cells were stimulated with different growth factor types, and fixed at various timepoints. Fixed cells were then stained with FoxO3, phospho-AKT, and phospho-ERK using standard immunofluorescent staining.