The ‘TranSeq’ 3’ End Sequencing Method for High-Throughput Transcriptomics and Gene Space Refinement in Plant Genomes

High-throughput RNA-sequencing technologies hold great value for exploring gene expression. Here, we describe ‘TranSeq’, a 3’ end-based sequencing approach enabling comprehensive transcriptome assays. Its application in plants is anticipated to upsurge the size of transcriptomics sample sets to the hundreds- and even the thousands- scale. TranSeq also outcompetes standard ‘full transcript length’-based RNA-seq methods in discriminating between highly similar transcripts. Mapping TranSeq reads to the current reference tomato genome improved > 45% of the gene models.