Gene expression profiles reveal the effect of a high-fat diet in brown fat development

Genome-scale analysis of the genetic factors that govern the development of white and brown adipose tissue is still far from complete. In order to identify the key genes that regulate the development of white and brown adipose tissue in mice, the transcriptome analysis was performed on adipose tissues Total RNA obtained from interscapular brown adipose tissue of C57BL/6J mice fed normal diet or high fat diet for 2, 4, 8, 20 and 24 weeks目前针对调控白色脂肪组织（white adipose tissue）与棕色脂肪组织（brown adipose tissue）发育的遗传因子所开展的全基因组尺度分析仍远未完备。为鉴定调控小鼠白色与棕色脂肪组织发育的关键基因，本研究对饲喂普通饲料（normal diet）或高脂饲料（high fat diet）2、4、8、20及24周的C57BL/6J小鼠肩胛间棕色脂肪组织（interscapular brown adipose tissue）提取的总RNA开展了转录组分析（transcriptome analysis）。