RNA sequence in H69AR cells

RNA sequencing in H69AR control group and SP8 siRNA group.RNA integrity was evaluated using a Bioanalyzer, ensuring only high-quality RNA was selected for library preparation. Following adapter ligation and PCR amplification, the libraries underwent sequencing on high-throughput Illumina platforms. Raw sequencing reads were subjected to quality control, aligned to the reference genome, and analyzed for gene expression, differential expression, and pathway enrichment. Additionally, the Illumina genetic algorithm was applied to optimize the pipeline for image processing and signal calibration. Gene expression levels were quantified in FPKM units, with differential expression defined as a minimum two-fold change and an absolute difference of at least 0.5 FPKM between groups.