Transcriptome analysis of zebrafish wildtype adult brain and rb1-embryonal brain tumors by RNA-Seq

Zebrafish wildtype adult brain and rb1-embryonal brain tumor transcriptomes were used to identify candidate rb1-interacting chromatin remodelers and epigenetic regulators altered in rb1- transformed brain tumor cells. The data was also used for comparative analysis with zebrafish rb1-/- mutant transcriptome, to identify molecular pathways that distinguish transformed rb1- tumor cells from non-transformed rb1-/- mutant cells. Overall design: RNA-Seq libraries were prepared from 10 tumor biological replicates and two pools of three normal adult brains ages 6.5 to 10.5 months.