Gene expression profiling of NUAK kinase overexpression in Drosophila larval muscle development

Signal transduction pathways mediated by kinases control diverse biological outputs at the level of cells and tissues to regulate a diverse array of biological and developmental events. To gain insight into how muscle expression of the evolutionarily conserved NUAK kinase regulates the transcriptional landscape during Drosophila melanogaster development, we performed high-throughput sequencing of RNA from either whole larvae or dissected muscle fillets at the end of larval development. Raw data was generated using the Illumina HiSeq platform. After trimming and mapping to the Drosophila reference genome, differential gene expression and GO enrichment analysis were completed. Comparative gene expression profiling analysis of RNA-seq data for mef2>lacZ control or NUAK (mef2>NUAK 548 or mef2>NUAK 550) overexpression samples. grant information: RO1 AR060788 Mechanisms Underlying Muscle Development and Maintenance in Drosophila, NIH/NIAMS