Tumor-infiltrating CCR2+ inflammatory monocytes counteract specific immunotherapy. Tumor-infiltrating CCR2+ inflammatory monocytes counteract specific immunotherapy

We used Single-cell RNA-sequencing to analyze the composition of the tumor microenvironment of MC38mOVA tumors upon transcutaneous immunization with our immunization method DIVA. Overall design: MC38mOVA tumor cells were inoculated s.c. into WT mice. Mice were treated by transcutaneous immunization at day 6/7 and 13/14 after inoculation or left untreated. At day 16, respectively day 20, single cell suspensions were prepared from excised whole tumors, and CD45+ cells were enriched by magnetic bead separation. Afterwards Single-cell RNA-sequencing of these CD45+ cells was performed.