Growth-rate dependence of parameters related to plasmid copy number control.

a. Estimated from MC using V = c×MC, based on the observation that cell mass and volume have the same growth-rate dependence (discussed in ref. [4]), using c = 0.2 µm3/(OD460 units/109 cells) [34]b. Lower values of the transcription rates have been reported for a different strain (2 min-1 for RNA I and 0.33 min-1 for RNA II at 0.9 dbl/hr) [28].c. The degradation rate has been reported to be independent of growth rate [25]. The absolute value used here is measured at 0.9 dbl/hr [22]. There are considerable differences between RNA I degradation rates measured in different labs, smaller values have been reported, ∼0.35 min-1, independent of growth rate [25] and ∼0.3–1.4 min-1, growth-rate dependent [26], see Text and Figure 3B.