Counting abortive transcription by Flux-seq. Counting abortive transcription by Flux-seq

To analyze abortive transcription rate close to TSS, we developed a new method called Flux-seq Overall design: All sample have been labelled by 4sU run-on and followed with biotin replace and enrichment. The original RNA have been fragmented for sequencing through. 3 run-on length: 5, 15,45mins have been designed and each time points have 3 replicates.