The ORFIUS complex regulates ORC2 localization at replication origins (Flow cytometry)

In this work, we use bromodeoxyuridine (BrdU)/propidium iodide cell cycle flow cytometry profiling to demonstrate that treatment of ovarian cancer cell lines with gene specific siRNAs, shRNAs, or drugs leads to minimal or no cell cycle arrest which might otherwise influence observed phenotypes. All raw FCS files for flow cytometry data presented in Figures 4E, 6G, S3A, S4C, S5B, S5F, S8C, S8D, S8E, S9C, S10C, and S12B are available here., The dataset was collected by treating different cell lines with either siRNAs or shRNAs targeting specific genes or drugs which perturb replication origins, treating with BrdU, and staining the cells with anti-BrdU antibodies and propidium iodide. The cells were then analyzed by flow cytometry. We are including here for each experiment FCS files for each individual sample run. 
siRNA transfection: 100,000 cells were plated in either a well of a 6-well plate or a 6 cm dish on day zero. On days one and two, cells were transfected with siRNAs by mixing 10 pmol of the appropriate siRNA with Lipofectamine RNAi/Max (LifeTech Cat. # 13778150) in Opti-MEM media (Gibco Cat. # 31985070) and adding the mixture directly to the cells. Transfected cells were utilized for various assays on day three or later.
Inducible hairpin experiments: OVCAR8 cells stably expressing control or BRD1 or HBO1 specific inducible hairpins along with either empty vector or shRNA-resistant BRD1 or HBO1 were assessed. Cel..., Any flow cytometry analysis software will open these FCS files. This would include FACS Diva or FlowJo., # Flow cytometry data for Yang and Hill ORFIUS complex manuscript

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In this work, we use bromodeoxyuridine (BrdU)/propidium iodide cell cycle flow cytometry profiling to demonstrate that treatment of ovarian cancer cell lines with gene specific siRNAs, shRNAs or drugs leads to minimal or no cell cycle arrest which might otherwise influence observed phenotypes. All raw FCS files for flow cytometry data presented in Figures 4E, 6G, S3A, S4C, S5B, S5F, S8C, S8D, S8E, S9C, S10C, and S12B are available here.

## Description of the data and file structure

The dataset was collected by treating different cell lines with either siRNAs or shRNAs targeting specific genes or drugs which perturb replication origins, treating with BrdU, and staining the cells with anti-BrdU antibodies and propidium iodide. The cells were then analyzed by flow cytometry. We are including here for each experiment FCS files for each individual sample run. The data is organized by Figure with one folder each for Fig...