Transcriptomic profiling of brassinosteroids response genes in soybean (Glycine max L.)

Brassinosteroids (BRs) are a series of sterol hormones in plants, which play important physiological roles in many aspects of plant growth and development processes. Exogenous application of BR and its inhibitors PPZ to investigated the roles of BR in soybean root growth and development. Transcriptome sequencing analysis was performed to identified lots number of differential expression genes(DEGs) by eBL (2,4-epicastasterone, 24-epiCS) and PPZ (propiconazole) treatment of soybean root tips(1 cm). Soybean seeds were germinated in vermiculite for 3 days. 3-day-old soybean seedlings were transferred to hydroponics medium for 5 days, then with 0.1 μM PPZ and mock treatment separately for another 2.5 days. 3-day-old soybean seedlings were transferred to hydroponics medium for 7 days, then treated with 0.01 nM eBL and mock for another half a day. PPZ_BR treatment is that 5-day-old hydroponic cultured soybean seedlings treat with 0.1 μM PPZ for 2 days and then with 0.01 nM BL for half a day.