Deng et al

在二倍体基因组中，基因有两个拷贝，它们可以有不同的 DNA 序列，一个是母本，一个是父本。在一个特定的细胞中，一个基因可能从两个拷贝（双等位基因表达）或仅一个（单等位基因）表达。我们对单个细胞进行了 RNA 测序，从受精卵到晚期胚泡的细胞，以及来自成年肝脏的单个细胞。使用两个远缘小鼠品系 CAST/Ei 和 C57BL/6 之间的第一代杂交，我们分别确定了母系和父系等位基因的表达。我们发现一半的基因仅由一个等位基因随机表达，因此一些细胞会表达父系等位基因，一些细胞会表达母系等位基因，少数细胞会同时表达等位基因。我们还观察到父系 X 染色体进行性失活的扩散。In a diploid genome, each gene carries two copies with distinct DNA sequences, one inherited maternally and the other paternally. In a given cell, a gene can be expressed from both copies (biallelic expression) or only a single copy (monoallelic expression). We conducted single-cell RNA sequencing on cells spanning developmental stages from fertilized zygotes to late blastocysts, as well as single cells harvested from adult mouse liver. Using first-generation (F1) hybrid mice generated from crosses between two genetically divergent strains, CAST/Ei and C57BL/6, we separately quantified the expression of maternal and paternal alleles. We found that half of all genes are randomly expressed from only one allele: some cells express the paternal allele, others express the maternal allele, and a small subset expresses both alleles. We additionally observed the progressive spreading of paternal X chromosome inactivation.

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞RNA测序技术，研究了小鼠从受精卵到晚期胚泡及成年肝脏细胞中母系和父系等位基因的表达情况。研究发现，一半的基因仅随机表达一个等位基因，并观察到父系X染色体进行性失活的扩散现象。