Summary of phenotypic screening results.

A summary of the top hits from the phenotypic screening of larval, juvenile and adult S. mansoni worms as well as adult S. japonicum worms for those hits that were successful in S. mansoni. Screen hits are shown, along with motility (as a percentage) and IC50 values (uM). The High Content Screening (HCS) larval assays were parametrised with positive controls with Oltipraz to define phenotype and mobility values with average scores of -0.66 for phenotype and -0.92 for mobility. The juvenile and adult assays followed a previously reported method which included appropriate positive controls (see Methods). To minimise animal usage, only where a ‘hit’ was obtained further screening was performed, starting with larval progressing to juvenile and then adult for S. mansoni then following up for adult S. japonicum.