RNA Sequencing Analysis of Human Non-Small Cell Lung Cancer Cell Line A549 in Dormancy Induction and Osteoclast-Activated States

When lung adenocarcinoma cells metastasize to the bone microenvironment, they often enter a dormant state. These dormant cells can be reactivated after bone microenvironment remodeling and reemerge as metastatic disease, posing challenges for the treatment and prognosis of lung adenocarcinoma. In the bone microenvironment, it is primarily osteoclasts that activate dormant lung adenocarcinoma cells. However, the specific mechanism by which osteoclasts contact dormant lung adenocarcinoma cells and promote their proliferation remains unclear. In this experiment, dormant lung adenocarcinoma cells were induced through in vitro culture and treated with osteoclast-conditioned medium for 24 hours to obtain osteoclast-activated lung adenocarcinoma cells. RNA-seq was performed on both dormant and activated lung adenocarcinoma cells to analyze the specific mechanism of the transition from dormancy to activation in lung adenocarcinoma cells following osteoclast interaction. In this experiment, dormant A549 cells and A549 cells activated by osteoclast conditional medium were collected, with three samples for each condition, totaling six samples for transcriptome sequencing. The goal was to compare the differential genes during the transition from dormancy to activation in lung adenocarcinoma cells.