Methylome description of CD4 T cells during chronic viral infection with either deletion of Dnmt3a, Tet2 or both Dnmt3a and Tet2.

The purose of this experiment was to determine how the loss of Tet2 and Dnmt3a altered the methylation program of CD4 Th1 and Tfh cells during chronic viral infection. Utilizing a SMARTA-Tg CD4 T cell adoptive-transfer model followed by LCMV-cl13 to induce chronic viral infection, spleens were collected at day 7 post-infection. After enrichment, SMARTA-Tg CD4 Th1 or Tfh cells were FACS Sorted into FACS Buffer. DNA was extracted using the Quick-DNA Micro-prep Kit (Zymo), and library construction with the NEBNext Enzymatic Methyl-seq kit was conducted. Samples were then sequenced.