Microbe abundance and biomass in the euphotic zone of the Aegean Sea at Stati...

10-30 ml subsamples were concentrated on 25 mm black polycarbonate filters (0.6 µm for NF, 0.2 µm for B), stained with DAPI for 10 min and filtered. NF were counted using UV and...将10~30 mL的子样品浓缩至25 mm黑色聚碳酸酯滤膜上，其中NF组使用0.6 μm孔径滤膜，B组使用0.2 μm孔径滤膜；随后用4',6-二脒基-2-苯基吲哚（DAPI）染色10分钟，再完成过滤操作。采用紫外（UV）对NF样本进行计数，后续内容省略。