Gene expression profile in endometrial organoids cultured in normal follicular phase vs PCOS-like hormone profile

We generated scaffold-free endometrial organoids from human primary endometrial tissues. These organoids were subjected to stepwise hormone treatments for 14 days mimicking the proliferative phase of menstrual cycle or with constantly high testosterone mimicking PCOS conditions Organoids from 4 patients were cultured in normal follicular phase and PCOS hormone profiles for 14 days and then subjected for RNAseq to determine genes differentially expressed in organoids cultured in PCOS condition.