T74-pT74-ALLO1-PRM
收藏NIAID Data Ecosystem2026-03-10 收录
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资源简介：
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by PRM analysis.
创建时间：
2018-01-31
