Experimental images and model depictions of organization of actin and FAs.

First column: DIC images of micropatterned triangular (A), square (F), and circular (K) myocytes. Second column: Immunostained actin in triangular (B) and square (G) myocytes followed the longest cellular dimension, while actin fibers in the circular myocyte (L) primarily oriented on the 2 to 8 o'clock axis. Third column: Predicted myofibrillar pattern of triangular (C), square (H), and circular (M) myocytes agrees with experimental results. The steady state of the circular cell occurred slower than that of the triangular and square cells. The thickness of the lines is proportional to the myofibril density . The grey color scale represents myofibril bundling, i.e. degree of parallel coupling . Fourth column: Immunostained vinculin of triangular (D) and square (I) myocytes was concentrated at cellular corners, while two opposing plaques of vinculin localized on the 2 to 8 o'clock axis in the circular (N) myocyte. Fifth column: Simulated FA density () at steady state in triangular (E), square (J), and circular (O) cells was consistent with experimental results. The FA distribution in a circular myocyte (O) was expected to break the symmetry. Color values in simulated results are in arbitrary units scaled from 0 to 1; scale bars are .