RNA-seq of High Grade Serous Ovarian cell line (OVCAR3) treated with ALDH1A1 inhibitor vs Control

Ovarian cancer (OC) is the leading cause of death from gynecologic malignancies in the US. Ovarian cancer stem cells (OCSCs) have been shown to drive chemoresistance and tumor progression but the mechanism remains incompletely understood. Aldehyde Dehydrogenase 1A1 (ALDH1A1) is a robust marker for cancer stem cells in ovarian and other cancers. We demonstrate that ALDH1A1 inhibition suppresses stemness, chemoresistance and senescence in ovarian cancer. Overall design: In triplicate, OVCAR3 cells were treated with compound 974 (5µM for 48h) or DMSO. RNA was harvested from the cells using the RNAEasy kit (Qiagen,Catalog No; 74106). RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina #RS-122-2101) according to manufacter's protocol.