Single-cell profiling of penta- and tetradactyl mouse limb buds identifies early mesenchymal progenitors required to specify digit numbers and asymmetry

Developing limb cells are heterogeneous. We used single cell RNA sequencing to compare a wildtype limb to two different Grem1 tetradactyl mutants at E10.75 and found differences in populations. Overall design: Limbs were chopped from E10.75 mouse embryos. Cells were dissociated and used to prepare libraries