five

Hodel et al Original Western blot data

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Original Western blot data used for Figure S3 in Hodel et al. 50 ug of cell lysate from the indicated cell lines were separated via SDS-PAGE and transferred to nitrocellulose membrane using standard conditions. The blot was then physically cut into sections and stained with the indicated primary antibodies, inlcuding p53, P-p53, Mdm2, p21, PUMA and GAPDH as a loading control. Longer exposures are also provided for Mdm2 and p53.
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2020-05-22
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