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Transcriptomic analysis of liver of WT and p21KO mice upon 24h fasting

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We profiled total liver mRNA of WT and p21KO mice that were fed ad libitum or fasted for 24 hours 2-3 mice of each genotype were either fed or fasted for 24 hours, sacrificed and total mRNA was extracted from liver (we mapped >12M reads per sample)

本研究针对野生型(WT)与p21基因敲除(p21KO)小鼠的肝脏总mRNA完成了表达谱分析。实验中每种基因型各纳入2-3只小鼠,分别设置为自由进食组与禁食24小时组,待处理结束后处死小鼠,提取肝脏组织总mRNA;本研究每个样本的比对测序读段(reads)数均超过1200万。

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