mRNA-Seq profile of gene expression in tip and stalk compartments of ureteric epithelium in developing mouse kidney

Morphogenesis of collecting duct system within developing mouse kidney is driven by growth at the tips of ureteric epithelium. To characterize the transcription program within the tip compartment, here we performed mRNA-Seq of tip cells (Wnt11RFP+;Hoxb7+ cells) and stalk cells (Wnt11RFP-;Hoxb7GFP+ cells) obtained from mouse embryonic kidney through FACS. We identified tip-specific genes from these data, and verified with in situ hybridization and followed up with mechanistic study for some of the interesting targets. Examination of two cell types within the ureteric bud of the developing mouse kidney发育中小鼠肾脏内集合管系统的形态发生，由输尿管上皮尖端的生长所驱动。为表征尖端区域内的转录程序，本研究通过荧光激活细胞分选（FACS）从小鼠胚胎肾脏中分离得到尖端细胞（Wnt11RFP+;Hoxb7+细胞）与柄部细胞（Wnt11RFP-;Hoxb7GFP+细胞），并对二者开展mRNA测序（mRNA-Seq）。本研究从上述数据中鉴定得到尖端特异性基因，通过原位杂交（in situ hybridization）对部分候选靶基因进行验证，并针对部分感兴趣的靶点开展了机制研究。针对发育中小鼠肾脏输尿管芽内的两种细胞类型的研究