Screen glycosyltransferase ST6GalNAc2 KO mice and B4GalNT1/B4GalNT2 double KO mice relative to C75 Wild Type controls

The purpose of this experiment is to respond to the NIGMS mandate to screen Core F generated glycosyltransferase KO mice in Core E and Core C. The goal is to screen several tissues for gene expression changes in ST6GalNAc2 KO mice and B4GalNT1/B4GalNT2 double KO mice relative to C75 Wild Type controls. Tissues for Core E analysis were selected by mining public databases including the NIH GEO database for tissues that expressed the KO genes at moderate to high levels. Results from Core E gene expression analysis are then used to help Core C select tissues to follow up with glycan analysis on in each of the KO mice. For the ST6GalNAc2 KO mouse we selected: Brain, Small Intestine, Lymph Node and Thyroid for analysis. For the B4GalNT1/B4GalNT2 double KO mouse we selected: Kidney, Mammary Gland, Thymus and Testes for analysis. Wild type tissues were analyzed in parallel.本实验旨在响应美国国家普通医学科学研究所（NIGMS, National Institute of General Medical Sciences）的任务要求，在Core E与Core C中筛选由Core F生成的糖基转移酶敲除（KO, Knock Out）小鼠。本实验的目标为，以C75野生型对照小鼠为参照，在多种组织中筛选ST6GalNAc2 KO小鼠与B4GalNT1/B4GalNT2双KO小鼠的基因表达差异。用于Core E分析的组织样本，通过挖掘包括美国国立卫生研究院（NIH, National Institutes of Health）基因表达汇编（GEO, Gene Expression Omnibus）在内的公共数据库筛选得到，筛选条件为该类组织中敲除基因呈中高水平表达。Core E的基因表达分析结果将用于辅助Core C选择组织样本，以在各KO小鼠中开展后续的聚糖分析。针对ST6GalNAc2 KO小鼠，我们选取了大脑、小肠、淋巴结与甲状腺开展分析；针对B4GalNT1/B4GalNT2双KO小鼠，我们选取了肾脏、乳腺、胸腺与睾丸开展分析；同时并行分析野生型组织样本。