RNA-seq of wheat leaves and roots in response to Xanthomonas translucens infection

This study aimed at decrypting the transcriptomic response of 2 months-old grown tender wheat (cv Chinese Spring) to a the Xanthomonas translucens pathogen infection by infiltration. The response was monitored by RNAseq 24h post leaf clipping. Triticum aestivum cv. Chinese Spring plants were maintained in a growth chamber with cycles of 12 h of light at 21°C and 50% relative humidity (RH) and 12 h of dark at 21°C and 50% RH. Leaves of 49 days-old plants were infiltrated with a bacterial suspension in water with an optical density at 600 nm (OD600) of 0.5 using a needleless syringe. Plants inoculated with water were used as controls. For transcriptomic and proteomic analyses, leaves and root tissues were harvested 1 day post-inoculation (dpi), when symptoms were not visible yet. Three biological replicates per treatment were performed, and each with pooled leaves from two independent plants per replicate. The files per conditions and replicates are:Sample 1 Root tissue with 3 replicates: CONTROL * control condition for roots (wheat without pathogen infection): 3 replicates: 1.1R,1.2R, 1.3R * control condition for leaves (wheat without pathogen infection): 3 replicates1.1L,1.2L, 1.3L * Wheat Roots infected by Xanthomonas translucens: 3 replicates: 5.1R, 5.2R, 5.3R * Wheat Leaves infected by Xanthomonas translucens: 3 replicates: 5.1L, 5.2L, 5.3L