Human STING Interactome During ER to Golgi Transport

The STING pathway is a central regulator of innate immunity that coordinates antiviral, antitumor and inflammatory programs. Upon sensing cyclic dinucleotides, STING exits the ER and traffics to the Golgi, yet how STING is preferentially packaged and delivered within the continuous stream of secretory cargo remains unknown. Here, we employed TurboID-based proximity labeling in living cells to capture the transient molecular environment of STING during ligand-driven transport. Affinity enrichment and LC-MS/MS were used to build a high-resolution map of STING-interacting proteins that govern ER-to-Golgi selectivity. These data clarify the basic cell-biological rules that license STING signaling and provide a comprehensive interactome resource for further mechanistic dissection.