Expression data from rat prefrontal cortex administrated with MK-801

Schizophrenia is a group of severe mental disorders. MK-801 is a common chemical that is used to construct schizophrenic animal model. In the present study, male SD rats were received MK-801 (0.2 mg/kg) intraperitoneal injections for 2 weeks. PFC tissue samples were collected freshly when sacrificing the rats and then quickly frozen and stored under -80°C for RNA sequencing. Differentially-expressed genes (DEGs) were determined by using DESeq2. A total of 129 DEGs, including 50 down-regulated and 79 up-regulated DEGs, were determined. By comparing with the online database TargetScanHuman (Release 8.0) and miRDB (Version 6.0), we found 4 genes (SIK1, TWIST1, BTG2, EGR2) that were altered in the schizophrenic model rat PFC and also are potential targets of miR-25.精神分裂症（Schizophrenia）是一组重型精神障碍。MK-801是一种常用化学试剂，常用于构建精神分裂症动物模型。本研究对雄性SD大鼠予以MK-801（0.2 mg/kg）腹腔注射，持续2周。大鼠处死后即刻采集前额叶皮层（Prefrontal Cortex, PFC）组织样本，随后快速冷冻并保存于-80℃环境中，用于RNA测序。采用DESeq2软件鉴定差异表达基因（Differentially-expressed genes, DEGs），最终共筛选得到129个DEGs，其中下调基因50个、上调基因79个。通过与在线数据库TargetScanHuman（Release 8.0）及miRDB（Version 6.0）进行比对，我们发现4个基因——SIK1、TWIST1、BTG2、EGR2——既在精神分裂症模型大鼠的前额叶皮层中存在表达异常，同时也是miR-25的潜在靶基因。