p25-bound CDK5 phosphorylates CDC25C

CDK5 activated by binding to p25 phosphorylates CDC25C protein tyrosine phosphatase at serine and threonine residues T48, T67, S122, T130, S168, and S214. CDK5-mediated phosphorylation of CDC25C interferes with 14-3-3-epsilon (YWHAE) mediated retention of CDC25C in the cytosol. Once activated by CDK5, CDC25C promotes activation of CDK1 (Chang et al. 2012).

CDK5通过与p25的结合激活，在丝氨酸和苏氨酸残基T48、T67、S122、T130、S168和S214处磷酸化CDC25C蛋白酪氨酸磷酸酶。CDK5介导的CDC25C磷酸化干扰了14-3-3-epsilon（YWHAE）介导的CDC25C在细胞质中的保留。一旦被CDK5激活，CDC25C促进CDK1（Chang等，2012年）的激活。