The effect of Neurensin-2 overexpression on gene expression in N2a cells

Neurensin-2 is a stress-responsive vesicular protein. Nonetheless, the cellular mechanisms by which Neurensin-2 regulates depression and anxiety remain unexplored. To identify the cellular pathways regulated by Neurensin-2, we overexpressed Neurensin-2 in a N2a neuroblastoma cell line, followed by RNA sequencing Overall design: To investigate the stress-induced gene expression changes, we extract RNA from control and overexpressed Neurensin-2 in a N2a neuroblastoma cell line .On the RNA, which was extracted from cells , we preformed sequencing, gene expression profiling and pathway analysis.