Proteomic Profiling of Pregnancy Day 8 Decidua in Uterine Localized Cadmium-Exposed Mice

This study investigates cadmium-induced proteomic alterations in gestational day 8 decidua using a uterine-localized exposure mouse model. Female ICR mice (7-8 weeks) received daily vaginal CdCl₂ (0.01 mg/mL, 100 μL) under isoflurane anesthesia (n=3 biological replicates). Tissues were snap-frozen in liquid nitrogen post-GD8 collection. Ultra Deep quantitative proteomics with diaPASEF acquisition (timsTOF Pro2, Wuhan Maiwei) revealed differential protein expression profiles associated with Cd exposure during early pregnancy.