Differential expression profiles of tsRNAs in myocardial ischemic rats with/without caloric restriction

We report the application of high-throughput RNA sequencing technology to examine expression profiling of transfer RNA (tRNA)-derived small RNAs (tsRNAs) in a normal control group (Norm group), isoproterenol (ISO)-induced myocardial ischemic group (MI group), and CR-pretreatment plus ISO-induced myocardial ischemic group (CR+MI group). A total of 714 precisely matched tsRNAs were identified in the myocardial tissues from the three groups of rats (701 in Norm, 708 in MI, and 712 in CR+MI). We find that 302 tsRNAs were identified to be notably dysregulated in the MI group: 166 tsRNAs were upregulated while 136 tsRNAs were downregulated compared with those in the Norm group. After CR pretreatment, we identified 136 tsRNAs that were significantly altered compared with those in the MI group: 45 were upregulated while 91 were downregulated. This study provides new ideas for future research on elucidating the mechanisms of CR pretreatment through regulating tsRNAs levels in myocardial ischemic injury.本研究采用高通量RNA测序技术，对正常对照组（Norm组）、异丙肾上腺素（isoproterenol, ISO）诱导的心肌缺血模型组（MI组）及CR预处理联合ISO诱导的心肌缺血模型组（CR+MI组）的转运RNA（transfer RNA, tRNA）衍生小RNA（transfer RNA-derived small RNAs, tsRNAs）表达谱进行检测分析。本研究在三组大鼠的心肌组织中共鉴定出714个精准匹配的tsRNAs，其中Norm组701个、MI组708个、CR+MI组712个。与Norm组相比，MI组中共鉴定出302个显著表达失调的tsRNAs，其中166个上调、136个下调。经CR预处理后，与MI组相比，共有136个tsRNAs发生显著表达变化，其中45个上调、91个下调。本研究为后续通过调控tsRNA水平阐明CR预处理抗心肌缺血损伤的作用机制提供了新的研究思路。