Cell death measured by annexin V assay and mitochondrial transmembrane potential (MTP) assay in endometrial cancer cell lines.

Endometrial cancer cells were treated with 100 μM of telmisartan for 48 h. Cells were then stained with annexin V and PI (propidium iodide). The positive cells were detected by flow cytometry. The viable cells were negative for both annexin V and PI staining (the lower left quadrant of the cytograms, LL), the early apoptotic cells were positive for annexin V staining but negative for PI staining (the lower right quadrant, LR), and the late apoptotic cells were positive for both annexin V and PI staining (the upper right quadrant, UR). MTP results were analyzed using the MitoCapture assay. Endometrial cancer cells were treated with telmisartan for 48 h and harvested for flow cytometry. Each experiment was repeated three times. Results = means ± SD of three independent experiments. *PP